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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been observed in many tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was uncovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital purpose in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two supplemental splice variants have been documented but detailed nucleotide sequence details isn't nonetheless out there.

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Blocking peptides are peptides that bind especially into the focus on antibody and block antibody binding. These peptide generally contains the epitope acknowledged by the antibody.

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This tutorial outlines more feasible optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...

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This guideline outlines additional feasible optimization of cellular and immunoassay parameters to ensure the very best outcomes are...

Antibodies bound to the blocking peptide no longer bind for the epitope around the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, you can see which staining is particular.

Concentrate on Facts Cyclin E belongs towards the extremely conserved cyclin relatives, whose members are characterized by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute into the temporal coordination of every mitotic event. Cyclin E forms a complex with and features to be a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress by way of S period.

The measurement of protein phosphorylation is a useful gizmo click here for measuring the modulation of receptor activation by the two antibodies...

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